Objective: To study the influence of compo un d Biejia Ruangan Prescription(CBRP) on extracelluar matrix in bleomycin induced
pulmonary fibrosis rats. Method: 54 male Sprague-Dawley r ats were randomly divided into 6 groups (9 rats in each group). Rats in the mode l control group, positive medicine group, and high, moderate and low CBRP groups were injected with bleomycin A5 by trachea, and rats in sham-model co ntrol group with same volume normal saline. 29 days after the injection, CBRP so lution of different dosages(1.4 g·kg -1 ,0.7 g·kg -1 ,0.35 g·k g -1 ) was respectively given to rats in the high, moderate and low CBRP gro up by gavage, while equal volume of normal saline was given to those in the sham -model control group and model control group, and an equal volume of prednisone (0.56 mg·kg -1 ) was given to those in positive medicine control group. On the 80 th day, the levels of Ⅲ-collagen,
Ⅳ-collagen, laminin and hyaluronic acid in the serum were determined, the determination of hydroxyproline in lung h omogenates was analyzed, and the right lung was incised to make pathological sec tions which were stained with Hematoxylin-Eosin(HE) and Masson staining for pat hological diagnosis. Result: CBRP coald decrense the levels of Ⅲ-collagen, Ⅳ-collagen, laminin and
hyaluronic acid in the serum. Conclusion: CBRP may play its therapeutic role by levelin g down the content of extracellular matrix in rats with pulmonary fibrosis induc ed by Bleomycin A5.sonstainingforpathologicaldiagnosis .Result :C