Objective To investigate the effects of Dansen on gastric mucosal Na+ K+ ATPase activity and gastric transmucosal potential
difference during severe
intraperitoneal infection in rats. Methods The intraperitoneal infection rat model was established by cecal ligation and puncture(CLP). Assay of Na+ K+ ATPase activity in gastric mucosal tissue was conducted by biochemistry method. The electric physiological recorder meter was used to measure gastric mucosal potential difference. Results The activity of Na+ K+ ATPase markedly decreased in infection group at 3 h after perforation, compared with the control group(P< 0.05). There was a minimum of Na+ K+ ATPase activity at 12 h after perforation in infection group (P< 0.01), was only 48.5% of that in the control group. At 12 h and 24 h post perforatively, Na+ K+ ATPase activity of Dansen group significantly increased as compared with infection group(P< 0.05). Gastric transmucosal potential difference (GTPD)in infection group decreased significantly as compared with the control group (P< 0.05) at 6 h after perforation, and rapidly dropped to the lowest at 12 h(P< 0.01). At 12 h and 24 h after perforation , GTPDs were higher in Dansen group than those in infection group(P< 0.05, P< 0.05). Conclusion The reduction of Na+ K+ ATPase activity may play an important role in gastric mucosal barrier damage following severe abdominal infection induced by CLP,and the application of Dansen in earlier period can prevent the occurrence of stress ulcer.