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Shvoong Home>Business & Finance>Determination of concentration of ropivacaine and bupivacaine in maternal and fetus plasma by revers Review

Determination of concentration of ropivacaine and bupivacaine in maternal and fetus plasma by revers

Article Review   by:TsingHua     Original Author: Chinese Journal of New Drugs and Clinical Remedies
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AIM: To establish reverse phase high performance liquid chromatography (RP-HPLC) method to determine the concentration of ropivacaine and bupivacaine in maternal and fetus plasma. METHODS: Agilent HPLC instrument was used with the column: Dikma-C 18(5 μm,4.6×150 mm). Internal standard was rosiglitazone. The mobile phase was composed of NaH 2PO 4(10 mmol·L -1,pH 3.0)∶CH 3CN(78∶22,V/V).Flow rate was 1.0 mL·min -1.Detection wavelength was 210 nm. RESULTS: The linear coefficient relation of ropivacacine and bupivacaine were Y=0.0295X- 0.0298 and Y=0.0287X+0.0271(r=0.9998,n=7). The minimum detection limit of ropivacaine and bupivacaine were both 0.01 mg·L -1. The linear ranges were both (0.01-5.0) mg·L -1. The average recovery rates of method were 99.82% and 101.01%. The coefficient variations of intra- and inter-day of ropivacaine for 0.1, 1, 5 mg·L -1 were less than 2.47% and 3.75%. The coefficient variations of intra- and inter-day of bupivacaine for 0.1, 1, 5 mg·L -1 were less than 2.69% and 4.75%. The concentration of sixty samples of ropivacaine was 0.15-0.7 mg·L -1, and that of bupivacaine was 0.1-0.58 mg·L -1. CONCLUSION: The RP-HPLC method is simple, sensitive and accurate. It is applicable to determine the concentration of ropivacaine and bupivacaine.
Published: June 05, 2004   
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