Objective:To study the influence of Astragalus
membranaceus injection on
lipid peroxidation(LPO) of human sperm membranes in vitro.Methods:Semen specimens of 30 patients with Asthenospermaia were exposed to Astragalus membranaceus injection in vitro experiment. The effects of Astragalus membranaceus injection on human
sperm motility characteristics were studied with computer-assisted sperm analysis (CASA) at 60min and 180min after treatment with injections. The Malondialdehyde(MDA) formation of the LPO of sperm membranes and the activities of superoxide dismutase(SOD) were determined at the same time in seminal plasma. The relationship between the LPO of sperm membranes and the sperm motility parameters was examined by linear regression analysis.Results:Astragalus membranaceus injection could increase percentage of viability (Mot), percentage of progressive motile sperm, the curvilinear velocity(VCL), average path velocity (VAP) and the lateral head displacement(ALH)(P< 0.05 or P< 0.01). MDA were significantly lower than those of controls(P< 0.05), while SOD were not significantly higher than those of controls(P> 0.05). There was a negative correlation between MDA and Mot, percentage of progressive motile sperm, VAP and ALH(P< 0.05 or P< 0.01).Conclusions:Astragalus membranaceus injection can inhibit lipid
Peroxidation(LPO) of human
sperm membranes in vitro, which provides experimental evidence for Astragalus membranaceus as an inhibiting
Lipid peroxidation drug for sperm perparation for ART.
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