Objective To study effect of Chinese herbal composition (Named
ZJKL powder) on twomarkers of epididymal function, glycerol-3-phosphorylcholine
(GPC) and sialic acid (SA). Methods An infer-tility model of male rat was established through putting GTW into rats stomach. And then the levels of spermmembrame GPC and SA in epididymal fluid were detected after being given ZJKL daily for 3 weeks. In thesame time sperm routine analysis of all groups were administered. Results ZJKL has obvious effect on levelsof GPC and SA. Contrary to the model group, ZJKL can significantly decrease level of GPC on the spermmembrane, and increase level of SA in epididymis fluid of male rat (P<0.05),especially, the low dosage grouphas more significant effect (P<0.01). There was same effect between ZJKL and the normal group (P>0.05).ZJKL can also obviously improve quality of sperm density and survival rate of spermatozoa, and lowerdeformity rate of spermatozoa (P<0.05). Conclusions Chinese herbal composition,
ZJKL powder, hasdefinite influence on markers of epididymal function of male rat. It suggested that male infertility can be curedby
traditional Chinese medicine through regulating epididymal function.