Objective: To investigate the difference of anticytomegaloviral activity of three kinds of traditional Chinese medicines
which are the injections of Jinye Baidu, Radix Isatidis and Indigowoa in vitro. Method:The inhibitory activity of three traditional Chinese medicines against
human cytomeglovirus( HCMV AD169) infected human embryo lung fibroblasts (HELF) was observed by cytopathic effect method (CPE) and MTT method in vitro. According their value of A , anticytomegaloviral activity has evaluated. Result:Experimental study in vitro showed that the 50% toxicity dose (TD_ 50 ) of Jinye Detoxifying, Radix Isatidis root and Indigowoa were 20, 10.23, 20.23 g·L -1 respectively;the 50% inhibitory concertration (IC_ 50 ) were 5.65, 3, 5.71 g·L -1 respectively;the therapeutic index (TI) were 3.54,3.41 and 3.54 respectively. It suggested that three traditional Chinese medicines had anticytomeglovirus activity and their effect increased with their concentration. Conclusion:Three traditional Chinese medicines of the parenteral solution of Jinye Detoxifying, Radix Isatidis root and Indigowoa have
antiviral activity when they are diluted in 1∶200. They are safe and valuable drug for inhibiting cytomeglovirus infection.