ObjectiveTo investgate effects of Chinese herb compound on
myocardial SDH,ATP-ase and
energy reserves in tail-suspended rats.
Method Male SD rats were randomly divided into three groups(n=10 each): (A) normal control group; (B)tail-suspended group; (C) tail-suspended + Chinese Medical herb compound group.Rats in group B and C were tail-suspended(-30°) for 5 d to simulate weightlessness. All rats B were dicapitated aftar the experiment.
Myocardial SDH(method of Anderson N-BT),ATP-ase(method of Wachstein and Meisel) and
energy reserves (method of HPLC)were examined. Result Compared with rats in groups A and C , SDH activity increased, SDH staining deepened,value of OD SDH enhanced,ATP-ase activity and OD value increased and enhanced signeficently(P<0.01)but value of myocardial energy reserves decreased in group B rats. Conclusion Tail-suspension strengthens compensatory oxidation metabolism of cardiac muscle and activats SDH and ATP-ase,at the same time decrease,myocardial energy reserves. Normal are maintained the low level of metabolism by the medical herb compound used which shows a protecting effects through mitigation,tranguilization and replenishment of Qi.