Phospholipid
vesicles on
polyelectrolyte multilayer shells can be stabilized against ethanol by coating a single
cationic polyelectrolyte. Confocal laser scanning
microscopy (CLSM) proved that the lipids were stabilized by cationic polyelectrolytes and the permeability to small hydrophilic dyes was decreased. Measurements of
fluorescence recovery after photo-bleaching (FRAP) with individual capsules enable quantification of release profiles.
From its conception a decade ago,
multiphoton microscopy has evolved from a photonic novelty to an indispensable tool for gleaning information from subcellular events within organized tissue environments. Its relatively deep optical penetration has recently been exploited for subcellularly resolved investigations of disease models in living transgenic mice. Its enhanced spectral accessibility enables aberration-free
imaging of fluorescent molecules absorbing in deep-UV energy regimes with simultaneous imaging of species having extremely diverse emission spectra. Although excited fluorescence is the primary signal for multiphoton microscopy, harmonic generation by multiphoton scattering processes are also valuable for imaging species with large anharmonic modes, such as collagen structures and membrane potential sensing dyes.
Keywords: Release; Vesicles; Phospholipids; Polyelectrolyte shells; Polymer complex; CLSM
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