Objectives: To evaluate the effects of traumatic stress on mice cellular immunity, and the influences of
Astragalus Polysaccharide
(APS) on the mice cellular immunity. Methods: A stress model of mouse amputed was established, and 50 Balb/C mice were divided randomly into 5 groups (10 mice each group): group A (normal control group), group B (traumatic stress group), groupC (traumatic stress+high dose APS group),group D (traumatic stress+middle dose APS group) and group E(traumatic stress+low dose APS group). The thymus and spleen of these mice stressed were weighted after 72 hours, and the expression of NF-闎 mRNA , IL-10 mRNA in lymphocytes from both tissues were detected by
situ hybridization. All results were quantitated by HPIAS-1000 system. Results: Compared with group A, the weight data were decreased significantly in group B (P<0.01), and the expression of NF-闎 mRNA and IL-10 mRNA in lymphocytes was increased evidently(P<0.01). Compared with group B, the weight data were elevated with different degree in groups C,D and E, and the expression of NF-闎 mRNA and IL-10 mRNA in lymphocytes were reduced (P<0.01). In group A and group C, the weight data and the expression of NF-B mRNA and IL-10 mRNA in lymphocytes of thymus and spleen had no significant difference (P>0.01>0.05). Conclusion: The mice cellular immunity function was inhibited after their trauma, but APS may restore the function effectively.