ObjectiveTo investigate the
inhibitory effects of
scorpion venom (SV) and its separated
components (SV Ⅰ、SV Ⅱ and SV Ⅲ) on the growth of B16
melanoma cells MethodsMTT method was used to detect the survival of
tumor cells treated with SV and its components in vitro and colony forming assay was applied to measure the
proliferation of tumor cells. Moreover, flow cytometry was further adopted to observe the changes of cell cycle of tumor cells ResultsSV Ⅰ, SV Ⅱ and SV Ⅲ could
Inhibit the growth of B16 melanoma cells significantly at the concentration of 200, 400 and 800 mg·L -1 ( P <0 05~ P <0 01) and SV could inhibit the growth of B16 melanoma cells at 400 and 800 mg·L -1 ( P <0 01~ P <0 05). Furthermore, SV and its components could inhibit colony forming of B16 melanoma cells ( P <0 01) at 400 and 800 mg·L -1 . Cells in G 0/G 1 stage were decreased and cells in G 2+M stage were increased obviously ( P <0 05~ P <0 01) after SV Ⅰ, SV Ⅱ and SV Ⅲ were given at 400 and 800 mg·L -1 , respectively.ConclusionSV and its components can inhibit the proliferation and survival of B16 melanoma cells. Moreover, the inhibitory effects of components are better than those of SV on B16 melanoma cells. Changes of cell cycle will be an important mechanism of inhibitory effects of SV.
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