Objective Effect of
Zhizhu decoction ( ZD) on mast cells( MC) of colonic mucosa in mice with
constipation due to spleen
asthenia was observed and the relationship between MC and the
immunoreactivity of substance P (
sp) and somatostatin (
ss) was explored to investigate the therapeutic mechanism of ZD.Methods The mice models with constipation due to spleen asthenia were induced by the discordance between starvation and engorgement plus overfatigue. The MC count and SP and SS expression were detected by modified toluidine blue method and LSAB immunohistochemical method respectively, and then analyzed with IMS color image processing system and related software. Results The MC density in the colonic mucosa in the constipated mice was reduced. The MC density was correlated positively with the mean intensity and area of SP immunoreactivity ( r=0.6508, P < 0.01; r=0.684, P < 0.01) and negatively with those of SS immunoreactivity ( r=- 0.7568, P < 0.01; r=- 0.683, P < 0.01) in the colonic mucosa. ZD in high or moderate dose could increase the MC density and the immunoreactivity of SP and decrease the immunoreactivity of SS. Conclusion The therapeutic mechanism of ZD for constipation due to spleen asthenia might be involved in the modulation of gastrointestinal motility via regulating the immunoreactive intensity of SP and SS and then influencing the release of 5- HT and histamine.
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