BACKGROUND:Renal fibrosis is almost the most common pathway of all the renal diseases from early period to telophase.However,the
majority of treatments on anti
renal fibrosis are still in the experimental period.Research showed that the traditional Chinese medicine has great prospect on anti renal fibrosis,and wenpi tang is an effective clinical agent for chronic renal failure and to protect renal function. OBJECTIVE:To explore the effect of wenpi tang on the function and morphology of remnant kidney of rats after 5/6 renal ablation. DESIGN:Randomized controlled experiment. SETTING,PARTICIPANTS and INTERVENTIONS:The experiment was completed in Cell and Biochemistry Laboratory of Beijing Traditional Chinese Medicine University.Forty four Wistar rats,male,weighing(200±20) g were selected and randomized into several groups according to their body mass before operation.Thirty six of them received 5/6 nephrectomy(wenpi tang group and niaoduqing group),and the rest 8 rats were in the pseudo operation.In 5/6
nephrectomized rats model,the effect of wenpi tang on bodymass,serum creatinine(Scr),blood urea nitrogen(BUN) and 24 hours' urine protein at different time point of pathological rats was dynamically investigated.Periodic acid methenaminesilver PAM staining result of renal tissue was studied by light microscope and image analysis technique in order to observe histomorphological changes. MAIN OUTCOME MEASURES:Comparison of serum creatinine,blood urea nitrogen,24 hours' urine protein and body mass before nephrectomy between pseudo operation group and other 3 groups. RESULTS:The serum creatinine<(9.4±1.0),(12.8±2.2)mg/L>of rats in wenpi tang group 12 and 16 weeks after operation was remarkably lower than that of model group<(11.7±1.7),(16.1±1.7)mg/L>,which indicated significant difference(t=4.052,P< 0.01).The serum urea nitrogen of wenpi tang rats increased gradually after operation,but it was still obviously lower than that of model group(t=6.504-15.498,P< 0.05) 12 and 16 weeks later.Twenty four hour urine protein of rats in wenpi tang and Niaoduqing groupsgradually went up while obviously lowered than that of model group at different time point(t=9.548-19.595,P< 0.01).Under light microscope,we found that wenpi tang alleviated the pathological damage such as glomerular hypertrophy,mesangial proliferation,glomerulosclerosis and tubulointerstitial damage. CONCLUSION:Wenpi tang can ameliorate renal pathological damage of 5/6 nephrectomized rats and protect renal function of the remnant kidney.