Objective:To probe into the transference of tinidazole across the blood-pancreas barrier in rats so as to provide theoretical
basis for the selection of appropriate antimicrobics for the treatment of secondary infections of the pancreas. Methods:35 SD rats served as the subjects of the study. Each of the animals was given a subcutaneous injection of tinidazole (20 mg·kg -1 body weight). Blood samples were taken 0,5,10,20,30,60,90,120,180 and 240 min separately after the injection and sera were separated. Pancreata of the animals were taken out separately at the same time points as mentioned above for the preparation of tissue
homogenates. The concentrations of tinidazole in the sera and
pancreatic tissue homogenates were determined with high performance liquid chromatography (HPLC). Results:The concentrations of tinidazole in sera and pancreatic tissue homogenates reached their peak values of 19.73 μg·mL -1 and 55.17 μg·g -1 20 min after the injection,respectively. The permeation ratio of the drug through the blood pancreas barrier was greater than 1. Conclusion:After a subcutaneous injection of tinidazole in the rat the drug was shown to have a fairly high distribution in the pancreas,denoting its good tranferability across the blood-pancreas barrier. It is therefore proposed by the authors that tinidazole may be used clinically for the prevention and treatment of pancreatic infections.