AIM:To develop an
LC-MS method for t he determination of
clarithromycin concentration in human plasmaMETH OD:20 μl of roxithromycin solution(10 μg/ml,internal standard)was a dded to 020
ml of plasmaThe
plasma samples were extracted with 4 ml ether a fter alkalizing using 01 mol/L Na 2CO 3Three ml of organic layer was transferred to clean tube and evaporated to dryness under N 2The res idue was reconstitued in 1 ml acetonitrile and 10 μl was injected into a n ODS C 18 (5 μm,250 mm×20 mm) columnThe mobile phase consisted of a cetonitrile:005% acetic acid (60∶40 v/v) at flow rate of 02 ml/min The eluate from the HPLC column was plumbed directly into ESI probe Analysis i n the
mass spectrometer was
operated in the selected-ion monitoring model The mass spectrometers was operated in SIM m/z 7485 for clarithromycin,837 5 for roxithromycin and 7645 for 14-hydroxylclarthromycin RESULT :Recoveries of clarithromycin at 0125,100 and 800 μg/ml were 865%,989% and 985% respectively The standard curve was linear in th e rang e of 00625～800 μg/ml The relative standard derivation of intra-day a nd in ter-day was smaller than 17% CONCLUSION: The me tho d of LC-MS is suitable for pharmacokinetic study of clarithromycin in human pla sma
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