Objective:The effects of glossy ganoderma on immunological liver injury and immune function were investigated in mice. Methods: The model of mouse immunological liver injury was induced by bacillus calmette guerin (BCG)+ lipopolysaccharide (LPS); The model of mouse enhanced or inhibited delayed type hypersensitivity (DTH) was induced by cyclophosphamidum (Cy); The model of mouse lymphocyte proliferation was induced by ConA or LPS. Results:The glossy ganoderma in a large dose(0.9g/kg,qd×10d,ig)could significantly decrease the levels of serum ALT(P<0.01) and AST (P<0.05), reduce the spleen lesion(P<0.05)and the liver damage in mice; It was also found the drug could markedly on one hand (0.3,0. 6,0.9g/kg,ig,qd×5d)inhibit enhanced DTH (P<0.05,P<0.01 and P<0.01,respectively.)and on the other hand (0.6,0.9g/kg,ig,qd×5d)enhance inhibited DTH(P<0.05 and P<0.01,respectively.) which induced by Cy. Moreover, the drug (0.3,0.6,0.9g/kg,ig,qd×5d)could inhibit T lymphocyte proliferation induced by ConA (P<0.05, P<0.01 and P<0.01, respectively.). Conclusion: These data suggested that glossy ganoderma could obviously regulate cellular immunofunction and have a protective action for immunological liver injury.