Cell microencapsulation is likely to play a major role in cell and transplantation therapies in the next decade. The microcapsules provide a special microenvironment in which cells always have different behaviors compared with free non-encapsulated culture. In this work, the
immobilized cells of the probiotic bacterium
b ifidobatrium bifidum No.1, 791 cultures on different carriers were investigated for the production of
amylase. amylase production by immobilized
bifidobatrium bifidum No.1, 791 cultivated in liquid media containing soluble starch reached a maximum at 48h. The entrapped cells of
bifidobatrium bifidum No.1, 791 in calcium alginate showed the highest
enzyme activity (90 U mL-1). Immobilization matrix was optimized. In repeated
batch fermentation, the immobilized cells preserve their ability to produce consistently over 21 cycles and the activity remain between (90 and 95 U/ mL-1) throughout the cycles. Some properties of the
crude enzyme produced by immobilized cells of
bifidobatrium bifidum No.1, 791 in repeated batch were studied. The enzyme properties revealed that the optimum temperature for activity was 75ºC. The enzyme was stable for 2h at 60ºC, while at 60ºC, 70ºC and 90ºC, 4%, 13% and 38% of the original activities were lost, respectively. The optimum pH of the enzyme was 7.5. After incubation of crude enzyme solution for 24h at pH 7.5, a decrease of about 5% of its original activity was observed. The enzyme was strongly inhibited by Cu2+ and Mg2+ but less affected by Ca2+, Mn2+. The enzyme in 1M and 5M NaCl solutions the enzyme retained 70% and 47% of the original activity after 24h of incubation at 4ºC, respectively.
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