Molasses as Bifidus Promoter on
Bifidobacteria and Lactic acid bacteria growing in skim milkreyed M. reyed1*Genetic Eng.
& Biotechnology Research Institute, Mubarak city for scientific research and applied technology. New Borg El-Arab, Post code 21934, Alexandria , Egypt
Lactobacillus platarum no. 8P-A3 and
bifidobatrium bifidum No.1, 791 inoculated in pasteurized 12 % skim milk containing 5 % (w/w) molasses, fructose, or lactose were studied. The total numbers of
bifidobacteria were stable throughout the study period in both subjects, but lactobacillus numbers were less constant and stable. Samples were collected at 24 and 48 hand examined for (a) viability of bacteria, and (b) levels of fermentation end products (lactic and acetic acids) as measured by HPLC. Molasses supported growth of bifidobacteria and lactic acid bacteria similar to other dietary carbohydrates. Bifidobacteria were evaluated for their potential use in skim milk. Strains were grouped according to their growth rate in 12% reconstituted skim milk. Molasses supported and enhanced growth of bifidobacteria and lactobacillus more than other dietary carbohydrates. Acetic acid and lactic acid production were enhanced when bifidobacteria were grown in the presence of molasses.