Molecular Characterization of Vibrio Vulnificus Isolated From Oyster Farm in Marang

The random amplified polymorphic DNA (RAPD) – PCR was used to analyze the genetic diversity of five Vibrio vulnificus strains isolated from oyster (Crassostrea irredalei). Reproducible profiles of genomic DNA fingerprints were generated by RAPD – PCR using Wild Type Phage M13 primer (5’- GAG GGT GGC GGT TCT -3’). The RAPD amplification was performed using DNA Thermal Cycler. The RAPD – PCR products were run in agarose gel for electrophoresis. Genomic similarity and genetic distance pattern was examined using NTSYSpc (Numerical Taxonomy and Multivariate Analysis System) software package, version 2.1, Dice coefficient and Unweighted Pair Group Method of Clustering (UPGMA) was used to generate a dendrogram. In RAPD analysis, the Vibrio vulnificus isolates were characterized using primers wild-type phage M13. The primer produced bands ranging from 200-1800bp. Five Vibrio vulnificus could be divided into two main clusters. The first cluster comprised isolates V1, V4 and V5. The second cluster was consisted of V2 and V3.