The objective of this research was to find out the effects of long-term application of fertilizers on the main soil enzymes
activities in a
Brunisolic soil to provide the theoretic basis for soil fertilization.The
long-term fertilization experiment was set up from 1979 in the experimental field of the Collage of Land and Environmental Science,Shengyang Agriculture University.The soil is classified as a Brunisolic Soil.Eleven treatments of fertilization were chosen for the study.The results showed that: during the growth period of corn,soil invertase,neutral phosphatade,alkaline phosphatase had two peaks of concentration,while match the two stages of jointing and filling period.However,single peak of urease concentration was in the jointing stage,whereas that of hydrogen peroxidase was in the bell-mouthed period.Manure could increase hydrogen peroxidase,invertase and urease activies,but decrease phosphatases activity.Phosphorous fertilizer could increase hydrogen peroxidase and invertase activities,while nitrogen fertilizer could have inhibiting effects on hydrogen peroxidase,invertase and urease.Significant correlation was found among the soil enzymes activities or between activities of the enzymes,contents of the soil nutrients,and corn yields.In a word,fertilizing properly can build a suitable biochemical environment in soil,and then improve soil fertility and crop yield.