This experiment was done in the 26 years fixed position experiment of non-calcareous fluro-aquic soil during the year of 2002,12 treatments were designed: CK,N1 ,N2,M1,M1N1,M1N2,M2,M2N1,M2N2, NPK,NP and NK. The effects of long-term located fertilization on potassium changes of the non-calcareous fluro-aquic soil were studied. The results indicated that: A combined application of organic fertilizer and inorganic nitrogen can also improve the content of soil available phosphorous ,it can increased 95. 39-191. 18mg/kg compared with that of non-application fertilizer. A combined application of nitrogen and phosphate and potassium fertilizer, nitrogen and phosphate fertilizer, nitrogen and potassium fertilizer can all enhance the content of soil available phosphorous, it can increased 24. 46mg/kg,24. 92mg/kg and3. 25mg/kg compared with that of non-application fertilizer. The results of 23 years' located fertilizer experiment indicated that; the soil available phosphorous quality score of non-fertilizer and only application nitrogen was decreased sharply, it can decreased 50% times 3 years later than that of s before trial, it can decreased 2/3 times in 7 years later than that of before trial, it can continue to decrease until to 4-5mg/ kg. But the soil total phosphorous quality score maintain the level of 0. 40-0. 50g/kg all along, the organic phosphorous enhanced a little. The soil available phosphorous and organic phosphorous quality score were enhanced sharply in the soil of only application organic fertilizer and combined inorganic nitrogen fertilizer.