Before and after each irrigation, the mineral nitrogen concentration in 0~200 cm soil of maize/barley intercropping was determined in Hexi corridor of Gansu province, this research arranges the 3 level of nitrogen application (0,150,300 kg/hm2), 2 level of irrigation water (816,1 632 m3/hm2). The results showed that soil mineral nitrogen concentration was higher at 0~60 cm depth before irrigation, yet it decreased obviously after irrigation, furthermore, soil mineral nitrogen content was more affected by the first and second irrigation.With 150 kg/hm2 nitrogen application,the mineral nitrogen loss of barley strip were 136.06 kg/hm2 after 816 m3/hm2 water irrigation ,and 142.93 kg/hm2 after 1 632 m3/hm2 water irrigation,which account for 90.70% and 95.29% of nitrogen fertilizer application, yet maize strip were 95.28 kg/hm2 and 115.89 kg/hm2,which account for 63.52% and 77.26% of nitrogen fertilizer application. With 300 kg/hm2 nitrogen application, the mineral nitrogen loss of barley strip were 264.43 kg/hm2 after 816 m3/hm2 water irrigation, and 237.72 kg/hm2 after 1 632 m3/hm2 water irrigation,which account for 88.14% and 79.24% of nitrogen fertilizer application, maize strip were 163.06, 257.76 kg/hm2,which account for 54.35% and 85.92% of nitrogen fertilizer application. Among three irrigation,the mineral nitrogen loss was the highest at the second irrigation with 150 kg/hm2 nitrogen application,and the first irrigation with 300 kg/hm2 nitrogen application, yet the barley strip all were the highest with the first irrigation. It suggested that mineral nitrogen loss was first decided by nitrogen fertilizer rate, then irrigation rate.