Using three varieties of B. campestris, Hauarad (708), Maoshan-3 (714) and Youbai (715) as the maternal plants and one variety of
B. oleracea Jingfeng1 (6012) as paternal plants, crosses were made to produce interspecific hybrids through
ovary culture techniques. The
ovaries from the cross between B. campestris × B. oleracea (708×6012 and 714×6012) were
cultured and ovary culture was more effective in terms of seeds
obtained when ovaries were in vitro culture at 9 days after pollination (DAP). While for the cross of 715×6012 it was better when ovaries in vitro culture at 12 DAP. Among three cross combinations, the cross of 714×6012
showed best
response and 43 seeds per ovary were obtained. Of the media studied, the ovaries from the cross of 708× 6012 were cultured on MS media supplemented with 3.0 mg·L -1 6-BA+0.1 mg·L -1 NAA+0.5% activated carbon showed better response, and its rate of seeds per ovary reached 44.0%. While the ovaries from the other two crosses (714×6012 and 715×6012) showed best response with cultured on B5 media supplemented with 3.0 mg·L -1 6-BA+0.2 mg·L -1 NAA+0.5% carbon, and the rates of seeds per ovary reached 72.0% and 60.0%, respectively. All seeds obtained from the three cross combinations were cultured on the MS media supplemented with 1.0 mg·L -1 6-BA+0.05 mg·L -1 NAA, and the seeds from the cross of 715×6012 showed best germination response and the percentage of germinations reached 66.7%. The regenerated plantlets were obtained from these seedlings after culture on the MS media supplemented with 0.05 mg·L -1 NAA. Cytological study showed that these regenerated plants were all true hybrids of B. campestris × B. oleracea.
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