Marigold(
Tagetes patula L )
plants grown in the greenhouse were daily treated with different concentrations of anaerobically
digested chicken manure effluent(ADCME).Plants treated with 1 68%-3 36% ADCME produced the highest fresh weight of shoot and open flowers,but those with 0 56% showed the highest fresh weight of root Samples of leaf in the middle part of plants and root were taken for biochemical analyses at harvest The relative permeability of cell membrane,and content of Pi,and activities of peroxidase(POD),Mg 2+ ATPase,and Ca 2+ ATPase in both leaf and root,protein and pH and activity of polyphenol oxidase(PPOD) in root tended to be increased with treated concentrations of ADCME in a certain degree Plants having
higher fresh weight of shoots and more flowers showed higher contents of chlorophyll,carotenoid and Pi in leaf,and activity of PPOD in root,and activities of POD,Mg 2+ ATPase and Ca 2+ ATPase in both leaf and root Plants showing salt toxic symptoms had significantly higher relative permeability of cell membrane and activity of POD in root,and activities of Mg 2+ ATPase and Ca 2+ ATPase in leaf All the ADCME treatments showed obviously higher contents of K +,Ca 2+ and Na + and pH than the control in both leaf and root extracts It is concluded that the double stresses of NH + 4 and salt in ADCME resulted in the symptom of salt toxicity in marigold,but a suitable concentration of ADCME could minimize the detrimental effects and fertilize plants well