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Shvoong Home>Science>Agronomy - Agriculture>Effects of cryopreservation on seed vigor and viability of rlte,rape and Ckinese cabbage. Summary

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Effects of cryopreservation on seed vigor and viability of rlte,rape and Ckinese cabbage.

Article Abstract by: TsingHua    

Original Author: JOURNAL OF ZHEJIANG AGRICULTURAL UNIVERSITY
The seeds with two levels of moisture content of rice(Oryza sativa L.),rape(Brassi-ca campestris var. oleifera)and Chinese
cabbage(Brassica chinensis L.)were cryopreserved in LN( liquid nitrogen,-196 ℃)for 5 or 7 days. The seeds packed in kraft paper or laminated aluminum foil packets were immersed directly from room temperature into LN and following storage were re-warmed rapidly in 38 ℃ water (rapid cooling/rapid rewarming)or rewarmed slowly in 1 8℃ room temperature (rapid cooling/slow rewarming).The results showed that the viability and vig-or of rape and Chinese cabbage seed were not influenced by cryopreservation, the viability of rice seed was not affected either, but the seedling growth of rice became weak significantly. The viabil-ity and vigor of rice and rape seed were not significantly different between rapid cooling/rapid re-warming and rapid cooling/slow rewarming;also the storability of three kinds of seeds was not remarkably different between higher and lower moisture content,however,in general trend, slow rewarming and lower moisture content were preferable to cryopreservation. There was not signifi-cant difference in rapeseed between kraft paper packet and laminated aluminum foil packet,but the former was better for rice seed and Chinese cabbage seed with higher moisture content.
Published: August 06, 1994
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