AIM To investigate the effects of vitamin E succinate ester(VES)on cytotoxicity of cisplatin (CP) and to explore the possibility of enhancing antitumor activity by the combination of VES and CP. METHODS Hepatocytes isolated from rats and humans by two step perfusions were used to evaluate cytotoxicity of CP and antiproliferative activity of CP in human prostate cancer cell line DU 145 and human colon cancer cell line CCL229; MTT assays were performed to evaluate cell viabilities. RESULTS Concentration of CP which inhibited 50% cell growth(IC50) was 2.35 and 4.70 mg·L-1 in primary cultures of human and rat hepatocytes, respectively. VES increased IC50 of CP to 6.93 mg·L-1 and more than 100 mg·L-1, respectively; IC50 of CP in DU 145 and CCL229 were 6.36 mg·L-1 and 9.58 mg·L-1, respectively. VES decreased IC50 to 0.58 mg·L-1 and 2.43 mg·L-1, respectively. CONCLUSION VES can decrease CP induced cytotoxicity in human and rat hepatocytes, and enhance antiproliferative activity of cisplatin in DU 145 and CCL229.