A test on competition in nodulation ability of peanut bradyrhizobia by AFLP fingerprinting method

The hereditary character and the nodulation competition ability, with the Tianfu No.3 peanut cultivar, were determined by AFLP fingerprinting method with Five p eanut bradyrhizobial strains of Spr2 9, Spr3 3, Spr3 5, Spr4 5, and Spr7 1. The results showed that: the variation of AFLP fingerprinting for all of the ex perimental strains cultivated at 28℃ as well as the strains of Spr3 3 and Spr3 5 incubated at 37℃ in YMB medium for 96 generations was not obvious, howeve r, the strains of Spr2 9, Spr4 5, and Spr7 1 could not grow at 37℃; the nodu lation competition rate was 85.4% for the strains Spr3 3 and Spr3 5 together, 12 2% for Spr4 5, 2 4% for Spr7 1, and zero percent for Spr2 9; Spr3 3 and Spr3 5 were thus the best strains due to the highest nodulation competition ra te an d well growth ability at 37℃. In general, the results suggested that AFLP finge rprinting was a good method to differentiate the studied strains, and it is an e asy, rapid and accurate method for the research on ecology and effective strains selection of Rhizobia.