A study was done to reveal the distribution of soil microorganisms and soil microbial stratification ratio which was based on the hypothesize that the degree of stratification of soil microorganism with soil depth,expressed as a ratio,could indicate soil quality or soil ecosystem functioning,in the ecological system at the crossing belt(Wuchuan County) of agriculture and animal husbandry.The results showed that the vertical distribution of total microbial number in 0～30 cm was 0～5 cm>5～12.5 cm>12.5～20 cm>20～30 cm,which decreased along with soil depth;there was no obvious difference in total microbial number among farmland,restored area and grazed grassland;and there was apparent difference in soil microbial stratification ratio among the three areas.And the conclusion of this paper was that soil microbial stratification ratio could be a good indicator of dynamic soil quality(the succeed direction of soil),ratio >2 would be uncommon under degraded conditions.Among the three areas,farmland had high risk of degradation;the restored area was on the procedure of restoration although the soil quality was still very poor;and the grazed grassland also had the risk of degradation but the speed was low.