Kosteletzkya virginica is a potential halophytic grain crop for consumption and industrial utilization.AFLP markers were
used to identify 36 F_1 hybrids of two parents.Eighteen primer combinations
amplified 1 100 AFLP bands between parent Hy103 and Hh182,108(9.82%) of which were polymorphic.Total 1 182 AFLP bands were amplified between parent Slhy353 and Blho301,148(12.51%) of which were polymorphic.Primer combination E+AAC/M+CTC amplified five paternal characteristic bands between Hy103 and Hh182:(401 bp),390 bp,156 bp,137 bp and 108 bp,whereas E+AAG/M+CTG generated seven paternal characteristic bands:457 bp,356 bp,312 bp,251 bp,240 bp,190 bp and 76 bp.All these paternal characteristic bands were found in eight F_1 hybrids of Hy103×Hh182,which were real hybrid.Primer combination E+AAC/M+CTT amplified five paternal characteristic bands between Slhy353 and Blho301:440 bp,393 bp,289 bp,162 bp and 67 bp,whereas E+ACA/M+CAT generated four characteristic bands:448 bp,360 bp,217 bp and 145 bp.All these paternal characteristic bands were found in 26 of 28 F_1 hybrids of Slhy353×Blho301,and other two F_1 hybrids were false hybrids.