Soil respiration is one of the most important biochemical processes that are relevant to the decomposition of soil organic
carbon and release of soil mineral nutrients. In this paper, we carried out an experiment of aerobic incubation using the Mollisols collected from the
long-term fertilization site starting from 1981 in Gonzhuling, Jilin Province. The treatments include different levels and combination of organic manure and mineral fertilizers. After incubation, we simulated the curve of the CO_2 released through the
soil respiration with incubation time according to the first order kinetic equation. The results indicated that the application of both organic manure and mineral fertilizers to soils could significantly increase the cumulative CO_2-C released through soil respiration, the potentially mineralized C content, and the ratio to total soil organic carbon and the mineralization rate constant. Long-term fertilization favored the release of mineral nutrients from the soil organic matter, thus increased the availability of soil mineral nutrients to crops and improved soil fertility status of the Mollisols. The interactive effect of organic manure and mineral NPK on soil respiration process was more significantly than that of other treatments.