Obviously, one of the ills plaguing the human race in a very devastating manner aside Human Immunodeficiency Virus (HIV), Malaria (Specifically to the tropics) and Tuberculosis (TB) is Hepatiits (inflammation of the liver). Among the organisms responsible for causing hepatitis, viral hepatitis remains enormous, easily transmittable and very difficult to treat. There are approximately 350 million people suffering from chronic Hepatitis B virus (HBV) infection worldwide (Kew et al, 1987). The HBV poses a serious threat to health personnel owing to the increased exposure to human body fluids of patients (Beutels et al, 2003) and also to patients from health personnel who may have the active or chronic infection of the HBV. This study was therefore undertaken to estimate the proportion of students of the College of Health Sciences (CHS) in Kwame Nkrumah University of Science and Technology (KNUST) in Ghana, who had active liver disease (ALT>80 U/L) due to HBV infection. Blood samples were drawn from 200 consenting students of the College. The following tests were done on the blood samples; ALT, AST level estimation and HBsAg determination. These were done to help estimate the prevalence of HBV infection and active liver disease among students of the CHS. 1% of the samples’ population presented with acute HBV infection. With such a lower prevalence of HBV infection and active liver disease among the students of the College of the Health Sciences, it is necessary that uninfected students are vaccinated to prevent lose of health personnel and possible transmission of hepatitis B virus to patients from infected health care workers.