A decade of dramatic advancement in analytical instrumentation and laboratory automation has shifted the analyst’s predominant tasks from assay measurement to sample preparation. As the requirements for higher sensitivity, selectivity , accuracy, precision and number of samples to be processed has escalated, the corresponding increase in speed and data collection and analysis has outpaced improvements in many traditional techniques of sample collection, preparation and quantification. High Performance Liquid Chromatography (HPLC) is one of such sophisticated techniques.
It has become the dominant analytical separation tool. It is used for the estimation of steroids, various polar drugs, amino acids and other biochemical samples. HPLC can be widely used for isolation and purification of complex carbohydrates, sugar, soft drinks and what not.