Using high-throughput technologies, abundances and other features of genes and proteins have been measured on a genome-wide
scale in Saccharomyces cerevisiae. In contrast, secondary structure in 5untranslated regions (UTRs) of mRNA has only been investigated for a limited number of genes. Here, the aim is to study genome-wide regulatory effects of mRNA 5-UTR folding free
energies. We performed computations of secondary structures in 5-UTRs and their folding free energies for all verified genes in S. cerevisiae. We found significant correlations between folding free energies of 5-UTRs and various transcript features measured in genome-wide studies of yeast. In particular, mRNAs with weakly folded 5-UTRs have higher
translation rates, higher abundances of the corresponding proteins, longer half-lives, and higher numbers of transcripts, and are upregulated after heat shock. Furthermore, 5-UTRs have significantly higher folding free energies than other genomic regions and randomized sequences. We also found a positive correlation between transcript half-life and ribosome occupancy that is more pronounced for short-lived transcripts, which supports a picture of competition between translation and degradation. Among the genes with strongly folded 5-UTRs, there is a huge overrepresentation of uncharacterized open reading frames. Based on our analysis, we conclude that (i) there is a widespread bias for 5-UTRs to be weakly folded, (ii) folding free energies of 5-UTRs are correlated with mRNA translation and turnover on a genomic scale, and (iii) transcripts with strongly folded 5-UTRs are often rare and hard to find experimentally.