Validation of the RSIDTM-Blood (Rapid Stain Identification of Human Blood) Assay Article Abstract
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Published: November 02, 2007
Validation of the RSIDTM-Blood (Rapid Stain Identification of Human Blood) Assay for the Confirmatory Identification of Human Blood by Seah Lay Hong, Normazlina Zainudin and Primulapathi Jaya
The RSIDTM-Blood Assay was validated in this study as a confirmatory Blood detection protocol for integration into existing standard laboratory procedures for DNA analysis in Kimia Malaysia. For this purpose the DNA Laboratory Extraction Buffer (formulated for use in standard extraction procedures in the laboratory) was compared with the RSIDTM-Blood Extraction Buffer provided with the RSIDTM-Blood Assay test kit. The DNA Laboratory Extraction Buffer was found to be more sensitive than the RSIDTM- Blood Extraction Buffer. The cross-reactivity and specificity of the RSIDTM-Blood Assay was tested with other body fluids (semen, urine and saliva) and with non-human blood sources (cow and fish).
The RSIDTM-Blood (Rapid Stain Identification of Human Blood) Assay uses two anti-human glycophorin A monoclonal antibodies in a lateral flow format to detect the presence of human glycophorin A. Glycophorin A is a 131-amino acid glycoprotein that spans the plasma membrane of human red blood cells and is expressed abundantly and specifically in red blood cell membranes where it is thought to prevent cellular aggregation. <1>. The RSIDTM-Blood is a confirmatory test for human blood and has numerous advantages over other methods of blood detection, including increased sensitivity, specificity, and speed <2>. Current identification methods for blood are presumptive (provide a basis for continued analysis of the tested exhibit but are not specific for human blood) and are sufficiently prone to false positives such that no definitive statement as to the origin of the tested stain can be made. Blood detection methods, such as the reduced phenolphthalein (Kastle-Meyer test) and ortho-tolidine tests are known to give false positive results with a variety of common substances including plant materials, detergents and scrap metal. Presumptive tests are inefficient, as they are poor predictors of DNA testing outcomes (and DNA testing is expensive) and are susceptible to legal and scientific challenges.
Buletin Kualiti dan Teknikal ; Bil. 11, Keluaran 1/2007
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