OBJECTIVE:To establish the analysis
method for
alpinetin and
cardamomin and evaluate the quality of Cao doukou (Alpinia katsumadai). METHOD: A HPLC method was deueloped by using a Shim Pack CLC ODS column(150mm×6mm). A mixture of methanol water (80∶20) was used as the mobile phase with a flow rate of 1 ml per minute and the detection wavelength was set at 300nm. The contents were calculated by external
standard method. RESULTS:The standard curves for alpinetin and cardamomin were linear over the range of injected amount of 0 05μg～0 26μg(r=0 9996 and 0 9998, respectively). The average recoveries of alpinetin and cardamomin were 101 9%(n=5, RSD=0 18%)and 100 0%(RSD=3 6%), respectively. Results of analysis of 5 samples show the contents of alpinetin and cardamomin in the semens of
Alpinia katsumadai were 0 50%～1 45% and 0 07%～0 72%
respectively and their contents in the pericarp, stem leaves and rhizome were less than 0 2%. CONCLUSION:The mothod is convenient, reliable and can be used for analysis of crude drugs.
More abstracts about the Quantitatve analysis of alpinetin and cardamomin in Alpinia katsumadai by HPLC