AIM To clarify the mechanism of
berberine on mobility diarrhea by investigating the effects of berberine on
calcium-activated potassium current I K(Ca) and
delayed-rectifier potassium channel current I K(V) of guinea pig
colonic smooth muscle cells. METHODS Single guinea pig colonic smooth muscle cells was isolated by collagenase; The effects of berberine on I K(Ca) and I K(V) were detected by using
patch clamp technique, under the conventional whole cell patch clamp mode. RESULTS 10, 50, 100 μmol·L -1 berberine inhibited I K(Ca) of guinea pig
Colonic smooth muscle cells significantly (P<0.05) when depolarizing at +80 mV,the I K(Ca) of 10, 50, 100 μmol·L -1 berberine were 68.20%±5.17%,55.89%±1.61%,48.08%±2.45% respectively compared with PSS group; 10, 50, 100 μmol·L -1 berberine inhibited I K(V) of guinea pig colonic smooth muscle cells significantly (P<0.05), when depolarizing at +80 mV,the I K(V) of 10, 50, 100 μmol·L -1 berberine were 77.06%±6.42%,68.67%±6.79%,61.07%±7.72% respectively compared with PSS group. CONCLUSION Berberine can inhibit I K(Ca) and I K(V) of guinea pig colonic smooth muscle cells, these may play an important role of its treatment effect on mobility diarrhea.