Objective:To observe the effects of
augmentin and
erythromycin on
pseudomonas aeruginosa biofilm in vitro.Methods:
polyethylene film and pseudomonas aeruginosa were co-cultured in LB medium for 48 hours until pseudomonas aeruginosa biofilm formed on polyethylene film.After that ,polythylene films were washed and transferred to LB medium containing different concentration of Augmentin and Erythromycin for further culture.polyethylene films were examined by scanning electric microscope to confirm if there were pseudomonas aeruginosa biofilms on it 24 hous later and LB media were vaccinated in LB agar plate to observe if pseudomonas aeruginosa were still alive in the cultured medium.Results:pseudomonas aeruginosa biofilm was formed on polyethylene film under scanning electric microscope after pseudomonas aeruginosa and polyethylene film were co-cultured in LB medium for 48 hours,howe ver,the biofilm disappeared after polyethylene film was cultured in media containing 100μg/
ml,300μg/ml,500μg/ml of augmentin and 50μg/ml,100μg/ml,300μg/ml of Erythromycin.Conclusion:pseudomonas aeruginosa biofilm can be formed after polyethylene film and pseudomonas aeruginosa were co-cultured in LB medium for 48 hours.Adequate high concentration of augmentin and erythromycin can kill pseudomonas aeruginosa covered with biofilm in vitro.