Objective: To investigate the effects of
ligustrazine on nitric oxide (NO), malonaldehyde (MDA) production, release of intracellular lactate dehydrogenase (
LDH) and
membrane fluidity of the injured human umbilical vein vascular endothelial cell line (ECV-304) with
hypoxia and lack of glucose. Method: The experiments were performed in culture of ECV-304 injured with
Hypoxia and
Lack of glucose in vitro. The released LDH of ECV-304 was measured with automatic biochemistry analyse. NO content of ECV-304 was monitored with colorimetry. Lipid peroxidation of ECV-304 was monitored as MDA with a fluorometric assay. The membrane fluidity of ECV-304 was measured with the fluorescence polarization method. Result: After culture ECV-304 in hypoxia and lack of glucose for 24 h, the LDH release, MDA production and the membrane fluidity increased significantly and NO level was decreased. Preincubation of ECV-304 with ligustrazine for 24 h reduced LDH release, MDA production, membrane fluidity increasing and increased the level of NO in ECV-304 due to hypoxia and lack of glucose. Conclusion: Ligustrazine has protective effect on injury of ECV-304 induced by hypoxia and lack of glucose.