The effects of
phenylalanine, sucrose and mannitol on the cell growth and the production of taxol, baccatin III and 10deacetylbaccatin
III in the suspension cells of
Taxus media were studied. The results showed that
phenylalanine 10 mmol·L-1 or 20 mmol·L-1 initially added into the medium, and sucrose 730 mmol·L-1 and mannitol 1733 mmol·L-1 added into the medium at the 28th d of culture strongly promoted the cell growth and the formation of the three taxanes in the suspension cells. Compared with those of the control, the cell biomass of the treatments supplemented with phenylalanine and added with sucrose and mannitol at the 28th d of culture increased by 06～08fold, taxol yield by 9～10fold, baccatin III yield by 25～30fold, and 10deacetylbaccatin III yield by 7fold. Addition of sucrose 730 mmol·L-1 at the 28th d of culture significantly promoted the cell growth, but showed little effect on the contents of the three taxanes in the suspension cultures.