BACKGROUND: Until now modern science cannot explain clearly about the mechanism of acute alcoholic liver injury. Besides such methods as giving up drinking, or symptomatic treatment, there is no specific therapy and remedy. Traditional Chinese Medicine (TCM) has obvious advantages for this: It cannot only improve the clinical symptoms, but also adjust organism immune function, and has very good development future. OBJECTIVE: To study the effects of
radix bupleuri and radix
scutellariae in compatibility with the ratio of different dosage (2∶1, 1∶1, 1∶2) on acute alcoholic liver injury by regulating the system of endocrine-immunity network in
rats. DESIGN: Groups are divided randomly and experiments are made under
blank conditions. SETTING: TCM Clinical Laboratory of Pharmaceutical School, Henan University of TCM. MATERIALS: From September to November 2002 in TCM Clinical Laboratory of Pharmaceutical School, Henan University of TCM, the general condition of the animals were as follows: 96 healthy Wistar rats were divided into 8 groups: blank group, model group, radix bupleuri group, radix scutellariae group, xiao-chaihu group, radix bupleuri-radix scutellariae 2∶1 group, radix bupleuri-radix scutellariae 1:1 group, radix bupleuri-radix scutellariae 1∶2 group. METHODS: ① According to the ratio of 2:1, 1:1, 1:2, radix bupleuri and radix scutellariae were taken respectively (480 g, 240 g; 360 g, 360 g; 240 g, 480 g) to produce the water decocting medicine. Except the blank group (duplicated by saline of the same volume through consecutive gastric infusion), the other groups are duplicated through consecutive gastric infusion by 56 degrees of Er-Guotou White Spirit (7 mL/kg) two times a day for ten days until the model-making is finished. From the first day, the medicine was given two times a day. The blank group and the model group were given the distilled water of the same volume. The other groups were given the proper water decocting medicine. The dosage in xiao-chaihu group was 10 g/kg and in the other was all 12 g/kg. ② 16 hours later after the last time when the white spirit was given, blood was taken from stomach aorta to produce serum. Liver, spleen, chest
gland, adrenal gland were quickly removed. The calculating formula of an organ
index: An organ index= The weight of an organ (g)/ The weight of a body (g) ×100%. The calculating formula of vitamin C content in adrenal gland (mg/g): Content of vitamin C= <(The A value of the testing tube-The A value of the blank tube)/ (The A value of the standard tube-The A value of the blank tube)> ×density of the standard tube (6 mg/L)×dilution times of the sample before tested/protein content (g/L). ③ Comparison between the groups was shown by using variance similar test and single-factor variance analysis. MAIN OUTCOME MEASURES: Index of rats' liver, spleen, chest gland, and adrenal gland (on the right side); the determination of vitamin C content in rats' right adrenal gland. RESULTS: During the experiment, 3 rats in the model group died. Two rats died in radix scutellariae group and radix bupleuri-radix scutellariae group respectively. In each of the other groups only one rat died. The condition of the number of the rats which were used for analyzing results: There were 12 in blank group; 9 in model group; 10 in radix bupleuri group, radix bupleuri-radix scutellariae 1:1 group respectively; 11 in each of the other groups. ① Comparison of index of rats in liver, spleen, chest gland, adrenal gland (right side) between different groups: There was no significant difference between the indexes of rats' liver in different groups. The index of spleen, chest gland, adrenal gland in the model group were all greatly less than in blank group (P < 0.05-0.01). The index of spleen in the radix bupleuri group were greatly higher than in the model group, it could make the reduced spleen index return to normal (P < 0.05); The index of chest in radix scutellariae group, radix bupleuri-radix scutellari
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