Objective To research the mechanism of
Danzhi-xiaoyao San(DZXYS) for treating
Alzheimer′s disease model of rats dealt with
D-galactose. Method An
Alzheimer′s disease-like model of rats has been set up with sc. D-galactose 150.0 mg·kg -1·D -1×49 d. Comparing with Acricept in 0.54 mg·kg -1·D -1 dosage as a positive control drug, DZXYS in 12.636 g·kg -1·D -1×49 d dosage has orally been administrated orally to treat the injury in the Alzheimer′s disease-like model of rats. The energy charge in the cerebral tissues had been detested with waters liquid chromatography; the protein content and DNA content in the cerebral tissues had been detested with ultraviolet assay, the relative content of aldose reductase-mRNA is detested with RT-PCR. The difference was analyzed between the control rats without D-galactose, the model rats dealt with D-galactose, the model rats treated with Aricept and the model rats treated with DZXYS, it is significant as P< 0.05. Result 1)DZXYS can not affact the energy charge in their cerebral tissues.2)DZXYS can increase the protein content from 0.3139±0.019468 to 0.3213±0.015528 (n i=10,P>0.05) in their cerebral tissues.3)DZXYS can increase the total DNA content from 1.093±0.267 to 1.488±0.341 (n i=10,P<0.01) in their cerebral tissues.4)DZXYS can increase the content of AR-mRNA in their cerebral tissue from 0.732±0.159 to 1.418±0.277(n i=5,P<0.01). Conclusion It suggests that DZXYS could be effective in human Alzheimer′s disease for its stabling gene expression, maintaining protein characteristics, recovering signal transduction in the Alzheimer′s disease-like model rats dealt with D-galactose.