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Influence of the Factor of Liver Depression and Spleen Deficiency on the Growth of Tumour and the I

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   by:TsingHua    
Original Author: Journal of Beijing University of Traditional Chinese Medicine
Objective To observe the influence of the factor of liver depression and spleen deficiency (LDSD) on the growth of tumour and the immune function of T cells in S180 tumor bearing mice. Methods Tumor cells were extracted from the abdominal cavity of S180 tumor bearing mice and adjusted to a concentration of 1×10 10 L -1 . The S180 tumor cells were inoculated into the Kunming mice by subcutaneous injection at the right oxter at a dose of 0 2 mL per mouse. LDSD syndrome was induced in the inoculated mice on the very day of the inoculation by the method of binding and an intemperate diet. At the same time, the single LDSD group, single S180 tumor bearing group and normal control group were also set up for the experiment. After the 10 th day of the inoculation, the mice were executed. The weight of tumor, the proliferation rate of T cells in the spleen, the ability to produce IL 2 and IFN γand the thymic index were measured. Results It was found that in the S180 tumor bearing mice, the LDSD factor could markedly increase the tumor weight ( P <0 01), decrease the proliferation rate of T cells ( P <0 01) and the ability to produce IL 2 and IFN γ( P <0 01), and lower the thymic index. Conclusion The LDSD factor can aggravate the suppression of cellular immune function and promote the growth of tumor in the tumor bearing mice.
Published: October 15, 2003
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