AIM To study influence of
ferulic acid sodium on the increase of endothelial
permeability induced by histamine. METHODS The endothelial cells isolated from bovine aorta were cultured on polycarborate microporous filter membrane to develop confluent
endothelial monolayer. Fluid filtration coefficient (Kf ), filtration volume ( Jv ) and osmotic reflective coefficient (σ ) to protein were measured in the
endothelial Monolayer treated by 1×10 -4 mol·L -1 histamine for 30 min or treated by 1×10 -4 mol·L -1 histamine for 30 min preincubated 1×10 -5 mol·L -1 ferulic acid sodium for 60 min after perfused either Hanks balanced salt solution or Hanks balanced salt solution containing 5 g·L -1 albumin. RESULTS Kf, Jv were increased after perfused Hanks balanced salt solution, and Kf , Jv were increased and σ decreased after perfused Hanks balanced salt solution containing 5 g·L -1 albumin in the endothelial monolayer treated by 1×10 -4 mol·L -1 histamine. Application of ferulic acid sodium in the endothelial monolayer treated with histamine induced reducing of Kf , and Jv after perfused Hanks balanced salt solution, and induced reduce of Kf , and Jv and σ enhance after perfused Hanks balanced salt solution containing 5 g·L -1 albumin, compared with in the endothelial monolayer treated with histamine purely. CONCLUSION Increases in the endothelial monolayer permeability induced by histamine, and ferulic acid sodium can inhibit increases in the endothelial monolayer permeability induced by histamine. The cellular mechanism of permeability response induced by ferulic acid sodium remain to be further elucidated.