Objective To study the effects of the
preconditioning with
enflurane and isoflurane on hepatic
ischemia and reperfusion
injury Methods Eighteen rabbits were randomly allocated to receiving no pretrearment (control
group, n=6),preconditioning with 1 68% enflurane (PE group,n=6),or preconditioning with 1 15% isoflurane(PI group,n=6), respectively, before undergoing 45 min of hepatic arterial and portal venous occlusions followed by 120 min of reperfusion The arterial blood samples were taken before the occlusion ,10 min and 120 min following reperfusion ,to measure the activities of serum alanine transminase (ALT), aspartate transminase (AST), lactic dehydrogenase (LDH), alkaline phosphatase (ALP) and gama glutaminase(GGT) using auto biochemical analyzer The liver histopathology were observed under light microscope Results Compared with those in control group , in PI and PE group the activities of serum ALT, AST, LDH, ALP and GGT were kept unchanged significantly before the ischemia (P>0 05); the increased degree of only AST activity reduced in PE group(P<0 05), others remained unchanged markedly in PI and PE group (P<0 05) 10 min following reperfusion; the increased degrees of all activities above reduced obviously in PI and PE group (P<0 05)120 min following reperfusion The injury degrees of liver histopathology in PI and PE groups were much less than that in control group Conclusions Preconditoning with enflurane and isoflurane can protect liver from ischemia and reperfusion injury to some extent