AIM To study the effect of L4oxalysine (OXL) on the
growth,
fetoprotein (AFP) secretion and
glutamyltranspeptidase
(GT) activity of human hepatoma
cells in vitro and in vivo. METHODS Antihepatoma activity in vitro was determined by MTT
method. Antihepatoma effect in vivo was studied in nude mice bearing human hepatoma. AFP
was assayed by ELISA and GT was determined by enzymesubstrate method. RESULTS After
using 086810-4molL-1of OXL for 6 d, the growth of the human hepatoma HepG2, Bel7402 and
Bel7404 cells cultured in vitro was slightly inhibited. At the concentration of 6810-4molL-1, the
inhibition rate was 137%456%. OXL 200 mgkg-1, po, qd2 wk could obviously inhibit the growth
of HCC93 human hepatoma transplanted into nude mice with inhibitory rate of 435% (P<005).
OXL significantly inhibited the secretion of AFP and the activity of GT of human hepatoma cells.
Mitomycin C at the therapeutic dose could inhibit the growth of human hepatoma cells and
decrease the GT activity significantly but produce slighter inhibitory effect on AFP secretion of
hepatoma cells. CONCLUSION The antihepatoma activity of OXL in vivo is stronger than that in
vitro and its mechanism of action was related to the inhibition of AFP secration and GT activity
of human hepatoma cells.