Objective To evaluate the inhibitive effect of combination of 3TC with Ara-AMP against HBV in vitroMethods Used 2.2.15
cell as target cell.With radioimmunological technique and blot slot,the inhibitive effect of 3TC,Ara-AMP and the combination of both against the HBsAg,HBeAg and intracellular HBV DNA were investigated.Results The inhibitive ratio of Ara-AMP against HBsAg,HBeAg was 45^48% and 41^46%
respectively when its
concentration was 400^0 μg/ml.Although 3TC also has inhibitive effect in its experimental concentration,its effect is weaker.When Ara-AMP 50^0 μg/ml was combined with 3TC 1^25 and 5.00 μg/ml respectively,the inhibitive ratio against HBsAg were 19^92% and 17^32% respectively.Compared with using same concentration 3TC alone,the difference of results was significant(P<0.05).But when compared with using the same concentration Ara-AMP alone,the difference of results had no statistical significance(P<0.05).Remarkable inhibitive effects of combination of 3TC with Ara-AMP against HBeAg were not found.When 3TC 5^00 μg/ml was combined with Ara-AMP 12^5 and 50^0 μg/ml respectively,the inhibitive ratio against HBV DNA was 45.90% and 50.36% respectively.Comparing the content of HBV DNA in these groups with that of control group and the groups using the same concentration 3TC and Ara-AMP alone,the differences were significant (P<0.05).Conclusion Combination of 3TC with Ara-AMP could enhance the inhibitive effects against HBV DNA.