AIM To study the effect of
Propylene Glycol Mannate Sulfate (Pgms) on induction of
CuZn SOD. METHODS Wistar rats were
given PGMS po at different doses (0, 18 9, 37 8 and 75 6 mg·kg -1 ·d) for ten days. Then the rats were sacrificed and the total RNA was extracted from the livers. The total RNA samples were loaded on a 1% agarose gel to detect the quality of total RNA. RT PCR was applied to study the expression of
CuZn SOD mRNA in rat livers. The amplified products were detected by the 1 5% agarose gel electrophoresis. Simultaneously, the CuZn SOD activities in rat liver were determined by nitrite method. RESULTS The total RNA extracted from rat livers was integrated without being decomposed by RNase. The level of CuZn SOD mRNA of the high dosage group (75 6 mg·kg -1 ·d) was higher than that of the control group (0 mg·kg -1 ·d) ( P <0 01); the CuZn SOD activities of the high dosage group were significantly higher than those of the control group ( P <0 001) and the CuZn SOD activities of the middle (37 8 mg·kg -1 ·d) and low dosage groups (18 9 mg·kg -1 ·d) were higher than those of the control group ( P <0 01). CONCLUSION PGMS can increase the CuZn SOD activities as well as CuZn SOD on mRNA level. Therefore, it is possible for PGMS to counteract Atherosclerosis (AS) by inducing the expression of CuZn SOD.