AIM To investigate
antiplatelet agents induced neuroprotection, which is blood perfusion independent, and its gender difference. METHODS Population spike amplitude (PSA) was measured during
hypoxia and posthypoxic recovery in blood fsee hippocampal
slices from untreated
control mice and from in vivo
pretreated mice with a single intraperitoneal injection of acetylsalicylic acid (ASA), ticlopidine or clopidogrel. Extracellular recordings and NADH fluorescence spectroscopy were used to determine PSA and NADH fluorescence upon hypoxia in CA1 region in hippocampal slices from ASA treated male and female mice. RESULTS Posthypoxic recovery of PSA was 25 9%±11 6% in control slices. In slices pretreated with ASA, ticlopidine and clopidogrel PSA recovered to 74 7%±35 7% ( P< 0 05), 67 6%±38 4% ( P< 0 05) and 77 8%±26 5% ( P< 0 01), respectively. Pretreatment with ASA resulted a different effect on neuroprotection between male and female animals. Posthypoxic recovery of PSA in slices from the with ASA pretreated female mice was 48 4%±34 0% ( P< 0 05 to female control mice, P< 0 05 to male pretreated mice ). A increase of hypoxic NADH reduced from 141 8%±9 9% in control slices to 117 9%±10 3% ( P< 0 05) in pretreated slices from male and from 204 9%±74 1% to 124 0%±11 0% ( P< 0 01) from female. CONCLUSION Blood perfusion independent pathway mediates part of neuroprotection due to antiplatelet drugs. This effect is gender depended. Part of the gender difference is mediated by different status of NADH related oxidative energy metabolism.