Objective To assess the effect of
intrathecal neostigmine and its possible mechanism in
formalin test. Methods Forty healthy male SD rats were randomly divided into control groups(NS, F)and test groups(NeF, LaNeF, LnNeF).
group NS served as normal control.Group F was treated with 5% formalin 100 μl, s.c. Group NeF, LaNeF, LnNeF were treated respectively with Neo 10 μg, L Arg 4.7 μmol
ith,L NAME 370 nmol ith before receiving 5% formalin 100 μl, s.c like group F. 0 1 h and 2 h after formalin treatment, behavior responses were observed and spinal cord segments were removed for
expression of Fos. Results The flinching and licking time of group NeF was shorter than that of group F, and the Fos expression of group NeF was weaker than that of group F. Pretreatment of L Arg or L NAME potentiated or reduced neostigmine induced responses respectively. Conclusion Intrathecal neostigmine could induce nitric oxide release in the spinal cord, the suppression of Fos expression might be one of the antinociception mechanisms of intrathecal neostigmine in the formalin test.
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