It was clearly demonstrated that T DNA of Agrobacterium tumefeciens Ti plasmid was integrated into the cells of crown
gall in our experiment. This paper reported the influences of some kinds of physical chemistry factors on the growth of crown gall of
Panax quinquefolium and the production of its main active compounds —— ginsenoside Re and ginsensoside Rg 1. The results showed that White medium was the best one for ginsensoside Rg 1 accumulation(0 095%) among the six media, but ginsensoside Re accumulation(0 194%) was the highest on the MS medium; The highest contents of ginsensoside Re(0 147%) and ginsensoside Rg 1 (0.061%) were on the culture 36d and 32d after innoculum respectively; The optimum pH was 5 6 for ginsensoside Rg 1 synthesis(0 054%), and 5 8 for ginsensoside Re synthesis(0 184%); The contents of ginsensoside Re and ginsensoside Rg 1 was the highest in the inoculum of 4g and 2g/flask(FW) respectively. The result also indicated that the concentration of inositol in 0 05g/L could obviously promote ginsensoside Re synthesis(0 182%), and in 0 30g/L for ginsensoside Rg 1(0 055%).