Aim To study the pharmacokinetic process about the concentration in rat plasma of the alkaloids from processed seeds of
Strychnos nux-vomica with RP-HPLC method. Methods Hypersil BDS C 18 column was used and the mobile phase consisted of acetonitrile-water at the flow rate of 0 8 mL·min -1 . The UV detection wave length was 254 nm. Results The concentration-time data of strychnine, brucine,
strychnine N-oxide and brucine N-oxide were all in accordance with an open two-compartment model after iv alkaloids. Their parameters were as follows: T 1/2 α were (8±5), (4±3), (6 2±1 7)and (3 0±0 8) min, T 1/2 β were (262±125), (416±131), (285±50) and (342±141) min, CL were (17±4), (21±12), (1 9±1 8) and (2 8±1 1) mL·min -1 , V c were (1 4±0 5), (1 7±1 1), (0 24±0 16) and (0 23±0 06) L·kg -1 , V d were (6 0±1 2), (12±7), (0 8±0 6) and (1 5±0 6) L·kg -1 , AUC were ( 57 578 ± 25 578 ), ( 35 240 ± 15 616 ), ( 93 088 ± 22 3 75 ) and ( 177 712 ± 120 110 ) h·μg·L -1 , respectively. Conclusion The method is a good reference for
pharmacokinetics in human bodies.